Fig. 5

Transcriptome analysis and tsRNA1 target gene prediction. (A) RNA-seq workflow. (B) Volcano plot of DEGs (fold change ≥ 2, FDR < 0.05). (C) Heatmap of DEG clustering. (D) GO analysis. (E) KEGG pathway enrichment. (F) Venn diagram of target gene overlap. (G) Proposed tsRNA1-mRNA interaction. (H) Predicted TET1 3’UTR binding. Note: Partial content created with BioRender (Wu, Y. (2024); BioRender.com/z82w555)