Skip to main content

A randomised feasibility tolerability study of aminophylline for the prevention of preterm labour

Abstract

Background

Progesterone is known to maintain uterine quiescence as pregnancy advances. Recently, its efficacy in preventing preterm birth has been questioned prompting a search for an alternative treatment option. Cyclic AMP has been shown invitro to act in synergy with progesterone to maintain uterine quiescence.

Methods

We undertook an open label randomised feasibility study to test the hypothesis that the addition of aminophylline to the standard of care (SoC) is acceptable and can be tolerated in pregnant women at high risk of spontaneous preterm labour (sPTL). Women at high risk of sPTL, who met the inclusion criteria were invited to participate and randomised to receive the SoC (progesterone alone, n = 33) or treatment with the SoC and aminophylline (n = 37). The main outcome measure was to assess the how many women at high-risk of sPTL tolerated and continued to take aminophylline. Data were analysed using Graphpad Prism 8.0c (Graphpad Software, San Diego, CA, USA).

Results

We found that of the addition of aminophylline was well tolerated in 30 of the 33 (91%) of women who continued in the combined arm, without any additional adverse maternal or fetal outcomes. 58% of eligible women agreed to participate in the study. The compliance rate was high at 99.42% +-0.82%. 67% of the women completed the post study questionnaire and all stated their willingness to take aminophylline if it were offered routinely for the prevention of sPTL.

Conclusions

The addition of aminophylline to the SoC is acceptable to women at high-risk of sPTL confirming that a randomised trial of aminophylline to reduce preterm delivery in women at high-risk of PTL is feasible.

Trial registration

Clinical trial gov NCT03152942. Date of full registration: 15/5/2017. https://clinicaltrials.gov/ct2/show/NCT03152942?cond=NCT03152942%26;draw=2%26;rank=1.

Peer Review reports

Background

Preterm birth (PTB) rates are increasing [1] and accounts for 11% of all pregnancies globally and 8% of births in the UK [2, 3] and is the leading cause of neonatal mortality. In women at high risk of PTB, progesterone (P4) supplementation is used clinically to reduce the risk of preterm labour (PTL) [4,5,6,7]. The OPPTIMUM trial, questioned the efficacy of P4 at reducing the risk of spontaneous PTB [8] and Blackwell et al. demonstrated that treatment with injectable 17-α-hydroxyprogesterone caproate did not reduce the risk of PTB among women high risk of PTB before 35 weeks [9]. The recent EPPPIC meta-analysis reported that vaginal progesterone (RR 0.78) and to a lesser extent oral progesterone (RR 0.60) reduced the risk of PTB before 34 weeks in high-risk women [10]. The conflicting evidence has prompted a call for research to be directed to alternative treatment options that may include adjunctive therapy to improve the effectiveness of P4 [11].

An alternative approach is to increase the intracellular levels of cyclic adenosine monophosphate (cAMP) in myometrial smooth muscle cells. cAMP is a potent smooth muscle relaxant with anti-inflammatory properties, which maintains myometrial quiescence during pregnancy [12] and is up-regulated by many endogenous pro-pregnancy hormones including relaxin, human chorionic gonadotropin and calcitonin gene-related peptide [13]. Therapeutically, increases in cAMP are commonly achieved by giving beta-adrenergic agonists like ritodrine or terbutaline. However, these drugs have severe cardiovascular side effects preventing their use in cases of PTL. Other approaches to increasing myometrial cAMP have been explored in cell, tissue and animal-based studies by inhibiting the metabolism of cAMP using a phosphodiesterase inhibitor. Our data suggest that this is a highly effective approach with fewer side effects [14]. We used aminophylline, a non-specific phosphodiesterase inhibitor, to increase intra-cellular cAMP, and showed that it reduces the response to IL-1b, and potentiates P4 action [15], inhibits myometrial strip contractility and potentiates the inhibitory action of P4 in a mouse model of PTL [14]. Further, animal and human data suggest that aminophylline improves perinatal outcomes, reducing the risk of respiratory distress syndrome, intraventricular haemorrhage [16,17,18] and bronchopulmonary dysplasia in extremely preterm infants [19]. Despite these beneficial effects, no formal studies have assessed the impact of maternally administered aminophylline on pregnancy outcomes.

Aminophylline is available as a modified release preparation called Phyllocontin Continus® which is given orally and contains aminophylline hydrate 225 mg. The medication is typically used for both children and adults to treat asthma and is also available in an intravenous preparation. The recommended oral dose in adults is 225 mg twice a day, which is increased after one week to two tablets per day [20, 21]. Aminophylline is rapidly metabolised to theophylline and peak serum levels are reached after 1.5–2 h of treatment, and toxic levels for humans are above 20ng/ml. Serum levels of theophylline are at or below 20ng/ml are associated with mild side effects such as nausea, vomiting, headache and insomnia. Side effects such as diarrhoea, irritability, restlessness, fine tremors and transient diuresis can also be reported at therapeutic levels between 10-20ng/ml [22]. Theophylline peak levels occur 1 to 3 h after oral ingestion of aminophylline and considered safe to use if breastfeeding is required [23]. Our objective was to undertake an open-label feasibility study to investigate the acceptability of oral aminophylline in women at high risk of PTB.

Methods

Study design

The study was an open label feasibility randomised study in a single centre. The study was approved by the East of England, Cambridgeshire and Hertfordshire Research Ethics Committee 16/EE/0299. Women attending the preterm birth surveillance clinic during October 2017 to March 2021 were screened for eligibility by the trial research midwife. Those who wished to participate and met the inclusion criteria were randomised to receive standard of care (SoC) alone or SoC with aminophylline. During the study, the trial management team, held monthly meetings to review the conduct and safety of the study and this meeting was also attended by the study patient and public involvement (PPI) lead. The study PPI group chaired by the PPI lead, held four meetings during the study and all participants completed an end of study questionnaire. Figure 1 summarises the CONSORT diagram.

Fig. 1
figure 1

CONSORT flow diagram for the trial showing participant flow through each stage of the feasibility randomised trial (enrolment, intervention allocation). P4– progesterone

Study population

Pregnant women who attended the prematurity clinic at the Chelsea and Westminster Hospital were assessed for study eligibility. All women with a singleton pregnancy were approached to participate if they were between 13 and 20 weeks of gestation, had a previous mid trimester loss (n = 19), previous spontaneous PTB (n = 28), short cervical length (n = 2), history indicated cervical suture (n = 18), ultrasound indicated cervical suture (n = 21), previous cervical surgery (n = 5), and previous mid trimester medical termination of pregnancy (n = 1, Table 1). Women were excluded if they reported any history of intolerance to aminophylline or if there were on any medications which would interact with the study Investigational Medicinal Product (IMP). The inclusion and exclusion criteria used are summarised in Table S1.

Table 1 Demographics of women and pregnancy outcomes in the study (excluding the withdrawals)

Study intervention

Standard of care (SoC) for high-risk women comprised a vaginal P4 with or without a cervical suture also inserted. Once recruited to our study, women were randomised to receive either receive SoC alone or with aminophylline. Aminophylline was issued from commercial supplies by Chelsea and Westminster Hospital pharmacy. Randomisation was done by the Chief Research Pharmacist using sealed envelopes. All IMPs were labelled for clinical trial use at the point of dispensing by the Chelsea & Westminster Hospital pharmacy department. Following randomisation, a clinical trial specific prescription was generated detailing the number of tablets necessary and instructions for use. This was based on a standard prescription template which was reviewed by the clinical trial monitor prior to recruitment. Prescriptions were fulfilled by the pharmacy and supplied to allow for compliance to be continually checked and to avoid wastage. The trial research midwife assured that the women received the study prescription and witnessed the first study drug dosing. Study visits, pharmacy supply, study questionnaire and observational components of the study comprised the following (Fig. 2):

Fig. 2
figure 2

Shows the study visits, observational components for each participant at each visit, and at which timepoint drug treatments were issued to participants. IMPs were either Aminophylline and vaginal progesterone (Am + P4) or vaginal progesterone only (P4)

  1. 1)

    Supply of the IMP was provided from baseline to week 22, week 22 to week 26, week 26 to week 30 and week 30 to week 34 of the pregnancy.

  2. 2)

    At the baseline visit, safety bloods (liver function, creatinine, urea, electrolytes, full blood count and clotting) were taken and concomitant medications checked.

  3. 3)

    At subsequent visits, safety bloods, tolerability, compliance with medications were checked at visits at 14, 16, 18, 22, 26, 30 and 34 weeks of pregnancy with a +/- 5-day window.

  4. 4)

    At 6 weeks after delivery, (+/- 1-week window) a final study visit was conducted, and a participant feedback questionnaire was completed.

  5. 5)

    Post randomisation the following medications were discouraged unless there is a clear and recognised indication: (i) Acyclovir, calcium channel blockers, cimetidine, erythromycin, clarithromycin, corticosteroids, and benzodiazepine carbamazepine and beta-sympathomimetics as they interact with aminophylline, and some can potentiate hypokalaemia.

  6. 6)

    No concomitant medications (prescription, over the counter or herbal) were administered during the study unless they were approved/prescribed by the Investigator for treatment of specific clinical events. All concomitant therapies were recorded on the case report form (CRF).

Study outcomes and data recording

Outcomes:

  1. 1)

    Primary outcome of the study was the number of women who withdrew due to side effects.

  2. 2)

    Secondary outcomes recorded were the number of women who:

    1. I.

      Were eligible to be recruited.

    2. II.

      Those who were willing to participate.

    3. III.

      Those who completed the study.

    4. IV.

      In those who did not complete the study, the reasons for not completing.

Additional data recorded: We also recorded the demographic data for the women, weekly caffeine consumption and both pregnancy, and neonatal outcomes.

Adverse reporting: Adverse outcomes (for example PTB and admission to neonatal unit) were anticipated due to the study population and all admissions to hospital were regarded as an adverse outcome and reported via the CRF. All admissions and pregnancy losses were reviewed by the trial monitoring team which included the chief investigator and principal investigator to determine if outcome was directly related to the study.

Sample size and statistical analysis

As this was a feasibility study a full power calculation was not performed. Data were analysed using Graphpad Prism 8.0c (Graphpad Software, San Diego, CA, USA). P values were two tailed and significance was defined as P < 0.05. For continuous data, data distribution was assessed using a Shapiro-Wilk test. Normally distributed data were analysed using a Student’s t-test for the two groups. Non-parametric data were analysed using the Kolmogorov-Smirnov test for the two groups.

Results

One hundred twenty women were screened and approached to participate in the study. 41 women declined participation (Table S2 summarises the reasons for the declining) and 9 did not meet the inclusion criteria. Of the 70 women who met the inclusion criteria and agreed to participate, 33 were randomised to SoC alone and 37 women were randomised to receive SoC and oral Aminophylline 225 mg twice a day. The demographics and baseline characteristics of the women in the study are summarised in Table 1. There was no statistically significant difference in the age, BMI, and gestation at recruitment between the two treatment groups. All the women in the study reported that they did not use caffeine throughout the study.

The primary outcome showed that 91% of women were able to tolerate aminophylline. Four women withdrew from the SoC arm, all for non-treatment related reasons, and 7 women withdrew in the SoC and aminophylline arm (3 for treatment related side effects and 4 for other reasons; Table 2). The median duration of aminophylline treatment before withdrawal was 5 days with range 1 to 39 days. All women who withdrew had a live birth at term.

Table 2 Reasons for withdrawal

Compliance outcomes

Overall compliance

Compliance for each participant was calculated via pack returns, patient diaries, and patient self-reports and reported as a proportion of the total doses of study medication used divided by the expected doses. Adequate compliance was taken as 80% of prescribed medication. For aminophylline, the median compliance was 99.42% +-0.82%.

Tolerability and side effects

Tolerability questionnaires were completed at study visits 14, 16, 18, 22, 26, 30 and 34 weeks of pregnancy. The participants were asked about the side effects that they experienced based on the 5 point Likert scale and whether it would affect a decision to take the treatment in the future [24]. Our findings are summarised in Table 1. In the SoC with aminophylline arm, more women reported side effects higher on the likert scale such as gastrointestinal upset, palpitations, headaches and skin rashes when compared to the SoC arm. The participants were asked if despite the side effects, they would accept the treatment in the future. Only two women in the aminophylline arm reported that they would not like to repeat the treatment in the future because of the palpitations and a skin rash they experienced respectively. In the SoC arm, two women reported depression/anxiety and one reported severe constipation.

Patient and public involvement outcomes

At the end of the study at 6 weeks postnatal, 63 participants who remained in the study, were sent a feedback questionnaire and 42 completed questionaries were returned (67% compliance with the questionnaire). 23 women in aminophylline arm and 19 in the SoC arm returned the feedback questionnaire (Table 3). In both arms of the study, 100% of the women who responded stated that they would accept the treatment in another study or if it became normal practice. However, it is important to note that the 3 women who did not tolerate Amniophylline did not complete the questionnaire.

Table 3 Participant feedback during the study

Regarding feedback on the overall participation of the study, 27 women said they would ‘definitely recommend’ a friend or relative to participate in the study. Thirty-four women reported that they were ‘extremely satisfied’ with the treatment arm they were allocated and only 2 reported that they were ‘fairly dissatisfied’. All the women reported that they were given enough information during the study.

Maternal outcomes

There was no statistical difference in the rates of gestational diabetes (GDM) or pre-eclampsia (PET), and the volume of blood loss at birth between the two treatment groups. In the SoC arm, there were 3 admissions for threatened PTL and one woman gave birth at 24 weeks. In the aminophylline group, there were 3 admissions for threatened sPTL and one woman gave birth at 33 weeks. There was one maternal admission to ITU for COVID-19 related reasons. Maternal outcomes are summarised in Table 1.

Fetal outcomes (live births, admission to NICU)

Fifty-three (76.7%) women in the study had a previous pregnancy. In the SoC arm, 15 women had a previous live birth and 9 had previous pregnancy loss. In the aminophylline arm, 15 women had a previous live birth and 14 had a previous pregnancy loss.

At the end of the study treatment, 28 women in the aminophylline arm had a live birth and two women had a pregnancy loss (at 19 weeks and 22 weeks of gestation). In the SoC arm, all 29 women had a live birth. There was one extreme preterm birth at 24 weeks in the SoC arm. Six women were in their first pregnancy (5 in SoC arm and 1 in aminophylline arm), and they all had a live birth. There were 5 cases of admissions to NICU in the aminophylline arm, related mainly to prematurity and the need for respiratory support. In the SoC arm, there were 3 admissions to NICU, two cases were for prematurity (24 weeks and 34 weeks) and the other at 38 weeks for reasons unrelated to the study medication since treatment with progesterone stopped at 34 weeks of gestation. The median stay for babies admitted to the neonatal unit was 17days+-21.7 days. Fetal outcomes are summarised in Table 1. At the end of the study, one of the babies born at 24 weeks was still an inpatient on the neonatal unit.

Outcomes on length of latency of pregnancy

We calculated the length of latency of the pregnancy among birth study groups. In.

SoC with aminophylline arm the length of latency was 10.05 weeks ± 7.9 weeks (n = 28) and in the SoC arm it was 9.63 weeks ± 7.2 (n = 24) weeks but this was not statistically significant (Figure S1).

Serious adverse events reported during the study

All admissions and pregnancy losses were treated as serious adverse events (SAEs) and reported and reviewed by the trial management team. In the aminophylline arm, there were 8 reported SAE and two in the SoC arm, and these are summarised in Table S3. None of the SAEs that were considered to be likely related to the study intervention were associated with any significant adverse maternal or neonatal outcomes. There was one intrauterine demise at 22 weeks in the SoC with aminophylline arm. This patient had a transabdominal suture, and the cause of death was due to sepsis. One woman in the SoC with aminophylline arm presented at 19 weeks with abdominal pain and vaginal bleeding and miscarried. There were no pregnancy losses in the SoC arm. Two women in the aminophylline arm were admitted for COVID-19 infection.

Discussion

Main findings

Our main finding was that the addition of oral Aminophylline to SoC was well tolerated by women at high-risk of sPTB. Compared to SoC, the addition of oral aminophylline was not associated with any significant adverse maternal or neonatal outcomes. The women reported that if aminophylline were to be part of SoC they would accept the treatment.

Strength and limitation

A major strength is that is a randomised study among women at high risk of sPTL. Furthermore, the feasibility study had a recruitment rate of 58%. However, the limitation of this study is that it was a feasibility study and therefore not powered to show a reduction in preterm birth < 35 weeks.

Interpretation

Collectively, previous data suggest that a cAMP agonist administered alone or in combination with progesterone supplementation might delay, preterm birth and improve perinatal outcomes [14, 15]. The current study was a feasibility study and was not powered to investigate any other perinatal outcomes. There was no difference in the postnatal outcomes which in some cases may be because we stopped the aminophylline at 34 weeks and 25 births occurred after this point. For this reason, we plan to continue the aminophylline until 36 weeks in the future study. Furthermore, given that aminophylline and caffeine are methylxanthines and have similar effects, the women in the study did not ingest caffeine and we are confident that caffeine consumption was not a confounder of the observations.

Preterm birth is not a single entity, rather it has multiple aetiologies and presenting with different phenotypes [25]. This may account for the conflicting response to P4 among women at an individual level. Our group has shown that P4 has anti-inflammatory effects and that these can be enhanced by the addition of a cAMP agonist in primary myometrial cells and in a mouse model of inflammation-induced PTL [14]. Equally, we failed to show any additive effect on in vitro myometrial strip contractility [15]. These data suggest that cAMP with progesterone may be most effective against idiopathic PTL, where inflammation drives the onset of labour.

This is a feasibility study not powered to show any difference in the outcome of pregnancy. In both arms of the study, 1 birth occurred between 24 and 34 weeks. Previous studies would suggest that in this high-risk population, 16–20% of women treated with progesterone would have been expected to deliver before 34 weeks [5, 8]. Had we seen a similar result in the current study, we would have expected 5–7 women to have delivered in the SoC arm as opposed to the 1 case that we saw. The unexpectedly low rates of preterm birth in the SoC arm limits our ability to perform any useful power calculation to inform a larger study of SoC with aminophylline vs. SoC alone.

Conclusions

This feasibility study showed that aminophylline is well tolerated and that subjects were highly compliant supporting its acceptability in this high-risk population and therefore supports the further investigation of the oral aminophylline in a double-blinded randomised controlled trial as a potential new treatment option for the prevention of sPTL and improvement in perinatal outcomes.

Data availability

All data generated or analysed during this study are included in this published article.

Abbreviations

cAMP:

cyclic adenosine monophosphate

CRF:

Case report form

IMP:

Investigational Medicinal Product

PPI:

Patient and public involvement

PTB:

Preterm birth

PTL:

Preterm labour

P4:

Progesterone

sPTL:

spontaneous preterm labour

SoC:

Standard of care

References

  1. Beck S, Wojdyla D, Say L, Betran AP, Merialdi M, Requejo JH, et al. The worldwide incidence of preterm birth: a systematic review of maternal mortality and morbidity. Bull World Health Organ. 2010;88(1):31–8.

    PubMed  Google Scholar 

  2. NHS Maternity Statistics England 2018–2019: NHS Maternity Statistics. 2019 [Available from: https://digital.nhs.uk/data-and-information/publications/statistical/nhs-maternity-statistics/2018-19

  3. Chawanpaiboon S, Vogel JP, Moller A-B, Lumbiganon P, Petzold M, Hogan D, et al. Global, regional, and National estimates of levels of preterm birth in 2014: a systematic review and modelling analysis. Lancet Global Health. 2019;7(1):e37–46.

    PubMed  Google Scholar 

  4. da Fonseca EB, Bittar RE, Carvalho MH, Zugaib M. Prophylactic administration of progesterone by vaginal suppository to reduce the incidence of spontaneous preterm birth in women at increased risk: a randomized placebo-controlled double-blind study. Am J Obstet Gynecol. 2003;188(2):419–24.

    PubMed  Google Scholar 

  5. Fonseca EB, Celik E, Parra M, Singh M, Nicolaides KH. Progesterone and the risk of preterm birth among women with a short cervix. N Engl J Med. 2007;357(5):462–9.

    CAS  PubMed  Google Scholar 

  6. Meis PJ, Klebanoff M, Thom E, Dombrowski MP, Sibai B, Moawad AH, et al. Prevention of recurrent preterm delivery by 17 alpha-hydroxyprogesterone caproate. N Engl J Med. 2003;348(24):2379–85.

    CAS  PubMed  Google Scholar 

  7. Romero R, Nicolaides KH, Conde-Agudelo A, O’Brien JM, Cetingoz E, Da Fonseca E, et al. Vaginal progesterone decreases preterm birth ≤ 34 weeks of gestation in women with a Singleton pregnancy and a short cervix: an updated meta-analysis including data from the OPPTIMUM study. Ultrasound Obstet Gynecol. 2016;48(3):308–17.

    CAS  PubMed  PubMed Central  Google Scholar 

  8. Norman JE, Marlow N, Messow CM, Shennan A, Bennett PR, Thornton S, et al. Does progesterone prophylaxis to prevent preterm labour improve outcome? A randomised double-blind placebo-controlled trial (OPPTIMUM). Health Technol Assess. 2018;22(35):1–304.

    PubMed  PubMed Central  Google Scholar 

  9. Blackwell SC, Gyamfi-Bannerman C, Biggio JR Jr., Chauhan SP, Hughes BL, Louis JM, et al. 17-OHPC to prevent recurrent preterm birth in Singleton gestations (PROLONG Study): A Multicenter, International, Randomized Double-Blind Trial. Am J Perinatol. 2020;37(2):127–36.

    PubMed  Google Scholar 

  10. Stewart LA, Simmonds M, Duley L, Llewellyn A, Sharif S, Walker RAE, et al. Evaluating progestogens for preventing preterm birth international collaborative (EPPPIC): meta-analysis of individual participant data from randomised controlled trials. Lancet. 2021;397(10280):1183–94.

    Google Scholar 

  11. Prior M, Thornton JG. Progesterone has no place in the prevention of preterm delivery: FOR: it is time to study something else. BJOG: Int J Obstet Gynecol. 2016;123(9):1510.

    Google Scholar 

  12. Yuan W, López Bernal A. Cyclic AMP signalling pathways in the regulation of uterine relaxation. BMC Pregnancy Childbirth. 2007;7(Suppl 1):S10.

    PubMed  PubMed Central  Google Scholar 

  13. Smith R, Parturition. N Engl J Med. 2007;356(3):271–83.

    CAS  PubMed  Google Scholar 

  14. Herbert BR, Markovic D, Georgiou E, Lai PF, Singh N, Yulia A, Johnson MR. Aminophylline and progesterone prevent inflammation-induced preterm parturition in the mousedagger. Biol Reprod. 2019;101(4):813–22.

    PubMed  Google Scholar 

  15. Lai PF, Young RC, Tribe RM, Johnson MR. Evaluating aminophylline and progesterone combination treatment to modulate contractility and labor-related proteins in pregnant human myometrial tissues. Pharmacol Res Perspect. 2021;9(4):e00818.

    CAS  PubMed  PubMed Central  Google Scholar 

  16. Melis GB, Fruzzetti F, Strigini F, Barale E, Capriello P, Baisi F, et al. Aminophylline treatment of preterm labor. Acta Eur Fertil. 1984;15(5):357–61.

    CAS  PubMed  Google Scholar 

  17. Hadjigeorgiou E, Kitsiou S, Psaroudakis A, Segos C, Nicolopoulos D, Kaskarelis D. Antepartum aminophylline treatment for prevention of the respiratory distress syndrome in premature infants. Am J Obstet Gynecol. 1979;135(2):257–60.

    CAS  PubMed  Google Scholar 

  18. Di Renzo GC, Mignosa M, Gerli S, Burnelli L, Luzi G, Clerici G, et al. The combined maternal administration of magnesium sulfate and aminophylline reduces intraventricular hemorrhage in very preterm neonates. Am J Obstet Gynecol. 2005;192(2):433–8.

    PubMed  Google Scholar 

  19. Schmidt B, Roberts R, Millar D, Kirpalani H. Evidence-based neonatal drug therapy for prevention of bronchopulmonary dysplasia in very-low-birth-weight infants. Neonatology. 2008;93(4):284–7.

    PubMed  Google Scholar 

  20. [http://www.evidence.nhs.uk/formulary/bnf/current/3-respiratory-system/31-bronchodilators/313-theophylline/aminophylline].

  21. Chen C-Y, Yang K-Y, Lee Y-C, Perng P-P. EFfect of oral aminophylline on pulmonary function improvement and tolerability in different age groups of Copd patients*. CHEST J. 2005;128(4):2088–92.

    CAS  Google Scholar 

  22. Nuhoglu Y, Nuhoglu C. Aminophylline for treating asthma and chronic obstructive pulmonary disease. Expert Rev Respir Med. 2008;2(3):305–13.

    CAS  PubMed  Google Scholar 

  23. NAEPP expert panel report. Managing asthma during pregnancy: recommendations for Pharmacologic treatment-2004 update. J Allergy Clin Immunol. 2005;115(1):34–46.

    Google Scholar 

  24. Sullivan GM, Artino AR Jr. Analyzing and interpreting data from likert-type scales. J Graduate Med Educ. 2013;5(4):541–2.

    Google Scholar 

  25. Singh N, Herbert B, Sooranna G, Das A, Sooranna SR, Yellon SM, Johnson MR. Distinct preterm labor phenotypes have unique inflammatory signatures and contraction associated protein profiles†. Biol Reprod. 2019;101(5):1031–45.

    PubMed  PubMed Central  Google Scholar 

Download references

Acknowledgements

The authors wish to thank all the women who participated in the study, the clinical and research staff at the Chelsea and Westminster Hospital who supported the study and the Borne Charity for funding the study.

Funding

The trial was funded by the Borne Charity.

Author information

Authors and Affiliations

Authors

Contributions

Conceptualisation and study design: MRJ conceived the study and NS and MRJ designed the study, and manged the clinical trial. Study set up, recruitment of women, data collection was done by MB, AY, GS, NMS. Data analyses were conducted by NS, MRJ and NMS. NS prepared the first draft with review and critical revision from all the other authors. All authors approved the manuscript prior to publication. Patient and public involvement was led by MB and NS.

Corresponding author

Correspondence to Natasha Singh.

Ethics declarations

Ethics approval and consent to participate

The study was approved by the East of England, Cambridgeshire and Hertfordshire Research Ethics Committee, reference 16/EE/0299, on the 5/12/2016. All experiments were performed in accordance with relevant guidelines and regulations of the Declaration of Helsinki. Informed consent was obtained from all participants in the feasibility trial.

Consent for publication

Not applicable.

Competing interests

The authors declare no competing interests.

Additional information

Publisher’s note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Singh, N., Shah, N.M., Sooranna, G. et al. A randomised feasibility tolerability study of aminophylline for the prevention of preterm labour. BMC Pregnancy Childbirth 25, 357 (2025). https://doiorg.publicaciones.saludcastillayleon.es/10.1186/s12884-025-07488-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doiorg.publicaciones.saludcastillayleon.es/10.1186/s12884-025-07488-1

Keywords